بررسی کارایی عصاره ناخالص آنزیمی استخراج شده از ریشه ترب کوهی درحذف کاتکول
محورهای موضوعی : تکنولوژی آب و فاضلابسهیلا امیری حسینی 1 , فاطمه نجات زاده 2 , اسماعیل بابانژاد 3 , فتح اله غلامی بروجنی 4 *
1 - دانشگاه علوم پزشکی مازندران
2 - گروه کشاورزی، دانشکده کشاورزی، دانشگاه آزاد اسلامی واحد خوی، خوی ایران
3 - دانشگاه علوم پزشکی مازندران
4 - عضو هیات علمی گروه مهندسی بهداشت محیط دانشگاه علوم پزشکی مازندران
کلید واژه: آنزیم, هورس رادیش پراکسیداز, کاتکول, فاضلاب سنتتیک ,
چکیده مقاله :
کاتکول یکی از ترکیبات فنلی است که بعنوان ماده خام اولیه و یا محصول نهایی صنایع شیمیایی، پتروشیمی و پالایشگاههای نفت می باشد. در این مطالعه بررسی کارایی عصاره ترب بعنوان آنزیم ناخالص و حذف کاتکول از فاضلاب صنعتی انجام گرفت. در یک مطالعه تجربی، کارایی حذف کاتکول از فاضلاب سنتتیک در مقیاس آزمایشگاهی در فاز ناپیوسته آنزیم ناخالص استخراج شده از گیاه ترب در فرایند حذف آنزیمی کاتکول مورد بررسی قرار گرفت. جهت انجام مطالعه کارایی فرایند های متغییر آنزیم ناخالص، غلظت های آب اکسیژنه در مدت زمان 20 دقیقه بررسی شد. پس از واکنش، غلظت کاتکول خروجی فرایند با دستگاه HPLC در طول موج 275 نانومتر اندازه گیری شد. با افزایش غلظت آب اکسیژنه و ثابت نگهداشتن غلظت آنزیم در ابتدا روند افزایشی مشاهده گردید. ولی پس از یک مدت زمان با افزایش بیشتر پراکسید هیدروژن تغییری محسوسی در راندمان مشاهده نگردید. به طوری که برای غلظت های آب اکسیژنه (0.58، 1.17، 1.78 مول بر لیتر ) و آب اکسیژنه (10، 20، 40 میلی لیتر بر لیتر) بترتیب راندمان حذف 45% ، 60% و 80% بدست آمد. ولی با مقادیر غلظت های آب اکسیژنه (1.17، 2.35، 3.53 مول بر لیتر) و غلظت آب اکسیژنه ثابت راندمان حذف بترتیب 42% ، 58% و 81% بوده است. تصفیه آنزیمی به عنوان یک فرایند مناسب می تواند جهت کاتکول و ترکیبات آن از فاضلاب مورد استفاده قرار بگیرد .
Catechol is one of the phenolic compounds (with chemical formula (C6H6O2) which is used as a raw material or final product of chemical, petrochemical and oil refineries. In this study, the efficiency of horseradish extract as a gross enzyme and removal of catechol from industrial wastewater was investigated. In order to study the efficiency of variable enzyme processes (20, 10, 40, ml / l), oxygenated water concentrations (0.58, 1.17, 2.35 mol / l) were evaluated for 20 minutes. The output catechol concentration of the process was measured by HPLC at a wavelength of 275 nm. The results of this study showed that with increasing the volume of oxygenated water and keeping the concentration of the enzyme constant, an increasing trend was observed at first, but after a while with a further increase in hydrogen peroxide, no significant change in efficiency was observed. For concentrations of hydrogen peroxide (0.58, 1.17, 1.78 mol / L) and hydrogen peroxide (10, 20, 40 ml / L), removal efficiencies of 45%, 60% and 80% were obtained, respectively. However, with the values of oxygenated water concentrations (1.17, 2.35, 3.53 mol / l) and fixed oxygenated water concentrations, the removal efficiency was 42%, 58% and 81%, respectively. Enzymatic treatment process as a suitable process can be used for catechol and its compounds from wastewater. To obtain the appropriate efficiency, it is better to set it in the optimal conditions under optimal conditions and add variables in the reaction.
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