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      • Open Access Article

        1 - Evaluation and comparison of some green methods for synthesis of silver nanoparticles
        sepide hamedi seyed abbas  shojaossadati
        Nowadays, there is an increasing need to develop high-yield, low cost, nontoxic, and eco-friendly procedures for synthesis of metallic nanoparticles. Therefore, the green synthesis methods become customary for synthesis of nanoparticles. Among metallic nanoparticles, na More
        Nowadays, there is an increasing need to develop high-yield, low cost, nontoxic, and eco-friendly procedures for synthesis of metallic nanoparticles. Therefore, the green synthesis methods become customary for synthesis of nanoparticles. Among metallic nanoparticles, nanosilver has developed because of its therapeutic properties. So in this paper, polysaccharide, tollens and biological green methods were investigated. In polysaccharide and tollens methods, starch and β-D glucose were used as a satabilizer and reducer respectively. In biological method biomass and cell filtrate of the Fusarium oxysporum fungus were used for the synthesis of nanoparticles. SEM images and UV-visible absorbtion spectra of these procedures showed that the polysaccharide method produced smaller silver nanoparticles wih high productivity. The changes of NADH-dependant nitrate reductase enzyme activity was evaluated in growh duration by colorometric Harely method due to importance of this enzyme in extracellular synthesis of silver nanoparticles. Results showed that the changes of dry cell mass impact on the enzyme activity. Manuscript profile
      • Open Access Article

        2 - The efficiency of crude enzyme extracted from horseradish root in oxidation of catechol
        Soheila Amiri Hosseini Fatemeh Nejatzadeh Eesmaeil  Babanezhad fathollah gholami
        Catechol is one of the phenolic compounds (with chemical formula (C6H6O2) which is used as a raw material or final product of chemical, petrochemical and oil refineries. In this study, the efficiency of horseradish extract as a gross enzyme and removal of catechol from More
        Catechol is one of the phenolic compounds (with chemical formula (C6H6O2) which is used as a raw material or final product of chemical, petrochemical and oil refineries. In this study, the efficiency of horseradish extract as a gross enzyme and removal of catechol from industrial wastewater was investigated. In order to study the efficiency of variable enzyme processes (20, 10, 40, ml / l), oxygenated water concentrations (0.58, 1.17, 2.35 mol / l) were evaluated for 20 minutes. The output catechol concentration of the process was measured by HPLC at a wavelength of 275 nm. The results of this study showed that with increasing the volume of oxygenated water and keeping the concentration of the enzyme constant, an increasing trend was observed at first, but after a while with a further increase in hydrogen peroxide, no significant change in efficiency was observed. For concentrations of hydrogen peroxide (0.58, 1.17, 1.78 mol / L) and hydrogen peroxide (10, 20, 40 ml / L), removal efficiencies of 45%, 60% and 80% were obtained, respectively. However, with the values of oxygenated water concentrations (1.17, 2.35, 3.53 mol / l) and fixed oxygenated water concentrations, the removal efficiency was 42%, 58% and 81%, respectively. Enzymatic treatment process as a suitable process can be used for catechol and its compounds from wastewater. To obtain the appropriate efficiency, it is better to set it in the optimal conditions under optimal conditions and add variables in the reaction. Manuscript profile